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Description History
SEPHADEX G-100 structure
Chemical Name:
SEPHADEX G-100;SEPHADEX(R) G-100;Sephadex G-100 60-100;Sephadex G-100 200-400;Sephadex G-100 100-200;SEPHADEX G-100 SUPERFINE;SEPHADEX G-100 DNA GRADE;Cross-inked dextran gel G-75;Sephadex G-100, Medium beads;Cross-inked dextran gel G-100
Molecular Formula:
Formula Weight:
MOL File:
Mol file

SEPHADEX G-100 Properties

Flash point:
38 °C
storage temp. 
room temp
2 - 10
  • Risk and Safety Statements
Risk Statements  10
Safety Statements  22-16
WGK Germany  3
HS Code  39139000

SEPHADEX G-100 price More Price(11)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich G100120 Sephadex? G-100 Medium 9050-94-6 10g $234 2019-12-02 Buy
Sigma-Aldrich G100120 Sephadex? G-100 Medium 9050-94-6 50g $899 2019-12-02 Buy
Sigma-Aldrich G100120 Sephadex? G-100 Medium 9050-94-6 500g $2740 2019-12-02 Buy
Sigma-Aldrich G10050 Sephadex? G-100 Superfine 9050-94-6 10g $234 2019-12-02 Buy
Sigma-Aldrich S6147 Sephadex? G-100 BioReagent, for molecular biology, DNA grade, medium 9050-94-6 25g $241 2019-12-02 Buy

SEPHADEX G-100 Chemical Properties,Uses,Production


Sephadex® G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography.
Sephadex G-100 is useful for desalting and buffer exchange of very large biomolecules.
  1. Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  2. Classic gel filtration medium.
Sephadex G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight. Smallest exclusion limit in the Sephadex range.
  1. Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  2. Suitable for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides larger than 700 molecular weight
  3. Efficient removal of contaminants such as salts, dyes, and radioactive labels
  4. Lowest exclusion limit (700 molecular weight) in the Sephadex G-range
Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency fractionation with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
Sephadex is a registered trademark of GE Healthcare


The introduction of Sephadex in 1959 heralded a new era in separation science. The concept of gel permeation chromatography (GPC) developed slowly and the key events leading to the discovery of Sephadex may be traced to Ingelman ?s early studies on the cross-linking of dextran, the subsequent patent by Flodin and Ingelman on the preparation of cross-linked gels and the exploitation of their gel filtration properties by Flodin and Porath.
Perhaps the most critical event was the synthesis of these gels in bead form with considerably improved flow rates, an innovation that led to the immediate acceptance of these gels in separation technology. Several types of Sephadex are currently available, each with a characteristic fractionation range. The most porous gel, Sephadex G-200, will fractionate proteins in the Mw range 4000–800000, whereas the upper limit for Sephadex G-25 is 5000. Although Sephadex gels are widely used for fractionation and purification of biopolymers, desalting constitutes one of the most important applications. In this context, desalting also refers to buffer exchange operations and the removal of low molecular weight impurities other than salts. Sephadex G-25 is now used for desalting or buffer exchange in many large scale operations. Insulin producers use Sephadex G-50 to remove proinsulin and protease impurities in the final stages of purification of porcine or bovine insulins.
The structure of Sephadex G-25 has recently been investigated by Holmberg (285) and the following novel structural features have come to light.
  1. Only 4% of the glucose units are cross-linked.
  2. Eight different structural elements were identified, including several glyceryl ethers of glucose and cyclic structures containing 1,4-dioxane rings. Two of these fragments are represented below 3 and 4.
  3. 80% of the beads consists of dextran.

SEPHADEX G-100 Preparation Products And Raw materials

Raw materials

Preparation Products

SEPHADEX G-100 Suppliers

Global( 42)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Shanghai Zheyan Biotech Co., Ltd.
18017610038 CHINA 3623 58
career henan chemical co
+86-371-86658258 CHINA 30043 58
Chongqing Chemdad Co., Ltd
+86-13650506873 CHINA 35434 58
future industrial shanghai co., ltd 400-0066-400
021-55660885; China 2000 65
Tianjin heowns Biochemical Technology Co., Ltd. 400 638 7771 China 14460 57
Hunan Hui Bai Shi Biotechnology Co., Ltd. 13308453923 0731-85529965-
0731-85526065;; China 3362 62
ShangHai LanJi Science and Technology Development Co., Ltd. 021-56373608/66107288/13391393747
021-66107288 China 279 62
ShangHai YuanYe Biotechnology Co., Ltd. 15800763013 021-61312847-
021-55068248 China 4976 60
Shanghai Ruji Biology Technology Co., Ltd. +86-21-65211385-8001 36031160
+86-21-65211385-8004 China 3228 55
Nanjing Dulai Biotechnology Co., Ltd. 18013301590 025-84699383-8004 China 898 55

View Lastest Price from SEPHADEX G-100 manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2018-12-25 Sepharose 2B
US $1.00 / kg 1kg 95%-99% 1000kg career henan chemical co

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